Jurkat cells were subjected to SDS PAGE followed by western blot with FNab05177(MICAL1 antibody) at dilution of 1:1000
IP result of anti-MICAL1(IP: FNab05177, 5ug; Detection: FNab05177 1:1000) with Hela cells lysate 2000 ug
Immunohistochemistry of paraffin-embedded human lung using FNab05177(MICAL1 antibody) at dilution of 1:100
Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using FNab05177 (MICAL1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L)
Background
Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization(Probable). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4.